Flow Cytometry Activity

Question 5

How could HIV be directly detected using flow cytometry?
Use an anti-HIV monoclonal antibody to label HIV-infected CD4+ T cells.
CORRECT!
HIV infects CD4+ T cells and expresses its proteins on the surface of the host cell. In this manner, anti-HIV antibodies can detect HIV infected cells which can then be analyzed by flow cytometry.


Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu

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